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Only diagrammatically explain the steps of rDNA technology
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0
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1790
|
September 2, 2016
|
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(i)Mention the number of primers required in each cycle of polymerase chain reaction.Write the role of primers and DNA polymerase in PCR
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1
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4838
|
September 2, 2016
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Any recombinant DNA with a desired gene is required in billion copies for commercial use.How is the amplification done ? Explain
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0
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1967
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September 2, 2016
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List the key tools and steps used in recombinant DNA technology
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0
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2456
|
September 2, 2016
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How is the amplification of a gene sample of interest carried out using Polymerase chain reaction?
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0
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966
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September 2, 2016
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(a)List the three steps involved in Polymerase Chain reaction
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1
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2128
|
September 2, 2016
|
|
(1)Mention the number of primers required in each cycle of polymerase chain rection(PCR). Writ the role of primers and DNA polymerase in PCR
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1
|
1008
|
September 2, 2016
|
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A anB are the two different cloning vectors in two different bacterial colonies cultured in a chromogenic subsrate.Bacterial colonies with cloning vector A were colourless, whereas those with B were blue coloured.Explain giving reasons the cause of the di
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0
|
925
|
September 2, 2016
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How can bacterial DNA be released from the bacterial cell for biotechnology?
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0
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497
|
September 2, 2016
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What is a primer?What is its role in PCR?
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0
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1733
|
September 2, 2016
|
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Name the type of bioreactor shown.Write the purpose for which it is used
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1
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2765
|
September 2, 2016
|
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(a)A recombinant vector with a gene of interest inserted within the gene of $\alpha $-galactosidase enzyme, is introduced into a bacterium.Explain the method that would help in selection of recombinant colonies from non-recombinant ones
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1
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2155
|
September 2, 2016
|
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How is insertional inactivation of an enzyme used as a selectable marker to differentiate recombinants from non-recombinants?
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0
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3448
|
September 2, 2016
|
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Why is the enzyme cellulase used for isolating genetic material from plant cells but not for animal cells?
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0
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2667
|
September 2, 2016
|
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How can bacterial DNA be released from the bacterial cell for biotechnology experiments?
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0
|
1230
|
September 2, 2016
|
|
Name the source of the DNA polymerase used in PCR technique.Mention why it is used?
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0
|
2722
|
September 2, 2016
|
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Write the names of the enzymes that are used for isolation of DNA from bacterial and fungal cells respectively for Recombinant DNA Technology
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0
|
2574
|
September 2, 2016
|
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Describe the characteristics a cloning vector must possess
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1
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2403
|
September 1, 2016
|
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The following is the flow chart highlighting the step in DNA fingerprinting technique. Identify a,b,c,d and f
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1
|
8946
|
September 1, 2016
|
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Draw a schematic sketch of pBR 322 plasmid and label the following in it :
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0
|
1450
|
September 1, 2016
|
|
Eco RI is used to cut a segment of foreign DNA and that of a vector DNA to form a recombinant DNA. Show with the help of schematic diagrams
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0
|
1966
|
September 1, 2016
|
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How are the following used in biotechnology?
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|
1
|
2293
|
September 1, 2016
|
|
Identify A and B illustrations in the following
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1
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1059
|
September 1, 2016
|
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Explain the basis on which the gel Electrophoresis technique works. Write any two ways the products obtained through this technique can utilized
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0
|
3781
|
September 1, 2016
|
|
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
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0
|
3266
|
September 1, 2016
|
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A recombinant DNA is formed when sticky ends of vector DNA and foreign DNA join. Explain how the sticky ends are formed and get joined
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0
|
2848
|
September 1, 2016
|
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State the role of DNA ligase in biotechnology
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0
|
1644
|
September 1, 2016
|
|
List the key tools used in recombinant DNA technology
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0
|
2570
|
September 1, 2016
|
|
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology
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0
|
1755
|
September 1, 2016
|
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Write the role of 'Ori' and 'restriction' site in a cloning vector pBR322
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0
|
3228
|
September 1, 2016
|