What is insertional inactivation?

what is insertional inactivation?

It is technique used in recombinant DNA technology in which a fragment of foreign DNA is inserted into a restriction site within a gene for antibiotic resistance, thus causing the genbe to be inactivated. The best example is pUC19 plasmid vector where the lacZ gene that encodes for beta - galactosidase cannot be longer produced when the foreign gene is inserted to it.