The data below were collected for an enzyme-catalyzed reaction.Determine.
Vmax and Km for this reaction.
Concepts and reason
The concept used in this question is of chemical kinetics. The kinetics of enzyme-catalyzed reaction is deeply discussed in Michaelis-Menten equation. According to this equation, rate of enzyme-catalyzed reaction is
Enzymes are biological catalysts. They possess incredible capacity in bringing about many complex chemical reactions in living organisms. They are highly specific in catalyzing chemical reactions.
In order to determine rate of reaction, one has to study the rate of decrease of substrate concentration with respect to time. In general, concentration of substrate will be studied with time to calculate rate constant or rate of any reaction.
From graph, intercept is measured as 0.2008.
From the graph, slope is measured as 0.5006.