List the steps of preparation of temporary mount of a leaf peel to observe stomata

CBSE Class 10 CBSE Class 10 Science
1

List the steps of preparation of temporary mount of a leaf peel to observe stomata.

2

The following are the steps of preparation of temporary amount of a leaf peel to observe stomata.

  1. Remove a healthy leaf from the plotted plant.
  2. Remove a part of the peel from the lower surface of the leaf by folding the leaf over and gently pulling the peel a part using forceps.
  3. Put a few drops of saffranin stain in a watch glass.
  4. After 2-3 minutes take out the peel and place it on a clean glass slide.
  5. Put a drop of glycerin over the peel and place a clean coverslip gently over it.
  6. Remove the excess stain and glycerin with the help of blotting paper.
  7. Observe the slide under the low-power and high-power magnifications of the compound microscope.