How is DNA recombinant technology helpful in detecting the presence of mutated genes in the cancer patients?
Molecular diagnosis in DNA recombinant technology uses a single-stranded DNA or RNA tagged with a radioactive molecules. It is allowed to hybridise to its complementary DNA in a clone of cells followed by detection using autoradiography. The clone having the mutated gene will not appear on the photographic film, because the probe used will not be complementary to the mutated gene. In this way, presence of mutated genes can be detected.