By seeing the V. S. of leaves, one of C3 plant and the other of a C4 plant.
(a) The C4 plant leaves have kranz anatomy.
(b) The bundle sheath cells make several layers around the vascular bundles.
(c) They posess large number of chloroplasts, thick cell walls impervious to gaseous exchange and there are no intercellular spaces e.g., maize and sorghum leaves.
(d) The chloroplasts in C4 leaves are dimorphic.
(e) The C3 plants do not have bundle sheath.
(f) They possess one type of chloroplasts.
(g) Photosystem I and II are present in each chloroplast.