You have created a recombinant DNA molecule by ligating a gene to a plasmid vector. By mistake, your friend adds exonuelease enzyme to the tube containing the
recombinant DNA. How will your experiment get affected as you plan to go for transformation now?
The experiment will not likely to be affected as recombinant DNA molecule is circular and closed, with no free ends. Hence, it will not act as a substrate for exonuclease enzyme, which removes nucleotides from the free ends of DNA.