Describe an experiment to prove that the nucleus is essential for the normal functioning of a cell?
- Nucleus is essential for carrying out the metabolic activities of the cell. It is also involved in cell differentiation.
- An experiment can be carried out by transplanting the nucleus from an irreversibly differentiated cell into an enucleated egg.
- The egg cytoplasm when normally nucleated is capable of giving rise to the complete range of differentiated cell types, while in the absence of the nucleus it may cleave but fails completely to differentiate.
- In other words, its differentiation, while potentially complete, is still nucleus-dependent.
- Therefore, if the egg nucleus could be replaced by one from a differentiated cell, the nature of the ensuing development should reveal the character of the transplanted nucleus.
- Complete differentiation would indicate that irreversible nuclear differentiation had not occurred, while limited differentiation would indicate that it had.
- This experiment suggest the crucial role of nucleus in cell differentiation.
- Likewise, several experiments can be formulated to establish the role of nucleus in cell functioning.