Write the role of ‘Ori’ and ‘restriction site’ in a cloning vector pBR322

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#1

Write the role of ‘Ori’ and ‘restriction site’ in a cloning vector pBR322.


#2

Ori is the site where replication starts. This site is responsible for controlling the copy number of a vector. Restriction site is the site of ligation of alien/foreign DNA in the vector, in one of the two antibiotic resistance sites or coding sequence of beta- galactosidase.