Write the bioassay test for gibberellin

plantgrowth
cbse

#1

The bioassay test for gibberellin are :
(a) Dwarf pea : Seeds of dwarf pea are allowed to germinate till the formation of coleoptile. GA solution is supplied to some seedlings. Others are kept as control. After 5-days epicotyl length is
measured. GA stimulates epicoty growth with a concentration as low as 1 nanogram.
(b) Barley endosperm: Endosperms are detached from efnbryos, sterlised and allowed to remain in 1 ml of test solution for 1-2 days. There is a build up of reducing sugars. The content of reducing sugar is proportional to GA cone. Reducing sugar are not ; formed endosperm in plain water