Where is NADP-reductase enzyme located in the chloroplasts? What is the role of this enzyme in
proton gradient development?
MADT reductase enzyme is located on the outer/stroma side of the thylakoid membrane. Along with the electrons from the primary acceptor of PS-I, it uses the protons of stroma for the reduction of NADP + to NADPH; this removal of protons from the stroma causes a proton gradient between the lumen of thylakoids and the stroma.