When an alien gene is ligated at the Pvu I site of ampicillin resistance gene in the vector pBR322, the recombinant plasmids lose ampicillin resistance due to insertion for the foreign DNA.
When an alien gene is ligated at the Pvu I site of ampicillin resistance gene in the vector pBR322, the recombinant plasmids lose ampicillin resistance due to insertion for the foreign DNA.