State role of DNA ligase in biotechnology

a. State the role of DNA ligase in biotechnology.
b. What happens when Meloidegyne incognitia consumes cells with RNAi gene?

a. Linking of DNA fragment is done by DNA ligase / linking of Okazaki fragments or discontinuous synthesis fragments / linking of desired gene with plasmid to form recombinant DNA.
b. Specific mRNA of the nematode silenced, parasite dies.