(i) How are recombinant vectors created?

cbse-class-12

#1

(i) How are recombinant vectors created?
(ii) For creating one recombinant vector only one type of restriction endonuclease is required. Give reason.


#2

(i) The vector DNA is cut at a particular restriction site using a restriction enzyme (to cut the desired DNA segment). The alien DNA is then linked with the plasmid DNA using an enzyme called ligase to form the recombinant vector.
(ii) Since a restriction enzyme recognises and cuts the DNA at a particular sequence is called recognition site, the same restriction enzyme is used for cutting the DNA segment from both the vector and the other source.