(i) How are recombinant vectors created?



(i) How are recombinant vectors created?
(ii) For creating one recombinant vector only one type of restriction endonuclease is required. Give reason.


(i) The vector DNA is cut at a particular restriction site using a restriction enzyme (to cut the desired DNA segment). The alien DNA is then linked with the plasmid DNA using an enzyme called ligase to form the recombinant vector.
(ii) Since a restriction enzyme recognises and cuts the DNA at a particular sequence is called recognition site, the same restriction enzyme is used for cutting the DNA segment from both the vector and the other source.