a. Describe the various steps of Griffith’s experiment that led to the conclusion of the ‘Transforming principle’.
b. How did the chemical nature of the ‘Transforming principle’ get established?
a. Streptococcus pneumonia
S -stain ‘inject into mice’ mice die.
R - strain ‘inject into mice’ mice alive.
S - strain ‘inject into mice’ mice alive.
R - strain + S strain ‘inject into mice’ mice die.
R strain transformed into virulent.
b. Purified biochemicals from heat killed S - strain.
Treated with protease - did not affect transformation.
Treated with RNase - did not affect transformation.
Treated with DNase - transformation affected.