Describe the characteristics a cloning vector must possess
CBSE Class 12
CBSE Class 12 Biology
2016-09-01 14:41:34 UTC
Describe the characteristics a cloning vector must possess.
Why DNA cannot pass through the cell membrane?Explain. How is a bacterial cell made'competent' to take up recombinant DNA from the medium?
2016-09-01 14:44:14 UTC
should have ori / origin of replication, - Has selectable marker, genes encoding fro an antibiotic resistance / genes encoding for - Has cloning site/recognition site, for the restriction enzyme to recognise.
DNA is a hydrophilic molecule.
Bacterial cell is made competent by treating with specific concentration of calcium ion /divalent ions, incubating them on ice, heat shock for a short period and placing it back once again.