An alien piece of DNA needs to be integrated to a specific sequence

cbse-class-12

#1

An alien piece of DNA needs to be integrated to a specific sequence of host DNA for its cloning. Is it true? if yes, explain. If this DNA is not picked up by host cells. Can you suggest a reason for it?


#2

Yes, for the multiplication of the alien DNA in the host, it has to be integrated to the origin of replication. The host cell is not competent hence it is not picking up the foreign DNA.